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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: J Biol Inorg Chem. 2015 May 7;20(5):805–819. doi: 10.1007/s00775-015-1267-1

Fig. 2.

Fig. 2

GdnHCl denaturation curves for the WT* (red circles) and WT*/K73H (blue squares) variants of iso-1-Cytc. Unfolding was monitored at 222 nm as GdnHCl was titrated into iso-1-Cytc buffered with 40 mM NaCl, 20 mM Tris buffer, pH 7.5 at 25 °C. Data in open symbols were not included in the fit to a two-state model (solid curves), described in the Materials and methods.