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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: J Biol Inorg Chem. 2015 May 7;20(5):805–819. doi: 10.1007/s00775-015-1267-1

Fig. 3.

Fig. 3

ε695corr vs pH data for the WT* (red circles) and WT*/K73H (blue squares) variants of iso-1-Cytc, which monitor the alkaline conformational transition of iso-1-Cytc. WT* iso-1-Cytc displays a monophasic transition (solid red curve is a fit to Eq. 1 in Materials and methods) and WT*/K73H shows a biphasic transition (solid blue curve is a fit to Eq. 2 in Materials and methods). Data were acquired in 100 mM NaCl at 22 ± 1 °C.