Table 1.

Thermodynamic parameters for iso-1-Cytc variants obtained by GdnHCl denaturation monitored by CD at 25 °C and pH 7.5.^a

Variant	ΔGu°′(H2O) (kcal mol−1)	m-value (kcal mol−1M−1)	Cm (M)
WT*	5.31 ± 0.08	4.39 ± 0.09	1.21 ± 0.01
yWTb	5.77 ± 0.40	5.11 ± 0.36	1.13 ± 0.02
WT*/K73H	4.09 ± 0.11	3.49 ± 0.12	1.17 ± 0.01
yWT/K73Hc	4.32 ± 0.12	3.59 ± 0.01	1.20 ± 0.03

^aParameters are the average and standard deviation of a minimum of three trials.

^bParameters are from ref. [49].

^cParameters are from ref. [33].