Fig. 3.

Neutrophil degranulation in response to Leishmania amastigotes. a Validation of a FACS-based degranulation assay. Thioglycollate-induced neutrophils were treated with cytochalasin B (CytoB, 5 mg/ml) for 10 min and then with fMLP (10 mM) for 4 h, resulting in a decrease in intracellular MPO staining. The MFI of MPO in treated samples was then compared to that of the controls (Med). b Neutrophil degranulation in response to amastigotes (MOI of 5) and/or LPS. *** p < 0.001, between indicated groups.