Table 3.
Comparison of Electronic Spectral Data for Nitrile Hydratase Enzyme vs Model Compounds
| λmax (ε) | ||
|---|---|---|
| MeCN | H2O | |
| [FeIII(DITpy)2]+ (1)a | 784 (1300) | 732 (1400) |
| [FeIII(DITIm)2]+ (2)a | 802 (1300) | 740 (1200) |
| [FeIII(ADIT)2]+ (3)b | 718 (1400) | 693 (1400) |
| [FeIII(AMIT)2]+ (4)b,g | 696 (1100) | |
| inactive enzyme (pH = 9)c,d | 690 (~1200) | |
| active enzyme (pH = 7.3)c,d | 710 (~1200) | |
| active enzyme (butyrate-free)e | 676 (NR)h | |
| enzyme + CH3CH2CNf | 690 (~4200) | |
a
This work.
b
Shoner, S. C.; Barnhart, D.; Kovacs, J. A. Inorg. Chem. 1995, 34, 4517–4518.
c
These spectra were recorded in the presence of a butyrate buffer.
d
Brennan, B. A.; Cummings, J. G.; Chase, D. B.; Turner, I. M., Jr.; Nelson, M. J. Biochemistry 1996, 35, 10068–10077.
e
Honda, J.; Kandori, H.; Okada, T.; Nagamune, T.; Shichida, Y.; Sasabe, H.; Endo, I. Biochemistry, 1994, 33, 3577–3583.
f
Sugiura, Y.; Kuwahara, J.; Nagasawa, T.; Yamada, H. J. Am. Chem. Soc. 1987, 109, 5848–5850.
g
Aqueous solution values not reported for complex 4 due to rapid decomposition in water.
h
NR = not reported.