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. Author manuscript; available in PMC: 2015 Jun 30.
Published in final edited form as: Inorg Chem. 2001 Mar 26;40(7):1646–1653. doi: 10.1021/ic001271d

Table 5.

EPR Parameters for Model Compounds vs Nitrile Hydratase

g values
[FeIII(DITpy)2]+ (1)a 2.16 2.10 2.02
[FeIII(DITIm)2]+ (2)a 2.19 2.15 2.01
[FeIII(ADIT)2]+ (3)b 2.19 2.13 2.01
[FeIII(AMIT)2]+ (4)b 2.20 2.16 2.00
[FeIII(PyAS)2]+c 2.17 2.11 2.01
[FeIII(PyMS)2]+c 2.13 2.09 2.03
[FeIII(PyPepS)2]+d 2.22 2.14 1.98
NHase enzyme + N3−e,g 2.23 2.14 1.99
NHase enzyme (pH = 9)e,f,h 2.20 2.12 1.99
NHase enzyme (pH = 7.3)e,f,h 2.27 2.14 1.97
NHase enzyme + (CH3)2CHCN (pH = 7.2)e,g 2.21 2.12 1.98
a

This work.

b

Shoner, S. C.; Barnhart, D.; Kovacs, J. A. Inorg. Chem. 1995, 34, 4517–4518.

c

Noveron, J. C.; Herradora, R.; Olmstead, M. M.; Mascharak, P., K. Inorg. Chim. Acta. 1999, 285, 269–276.

d

Noveron, J. C.; Olmstead, M. M.; Mascharak, P. K. Inorg. Chem. 1998, 37, 1138–1139.

e

These spectra were recorded in the presence of a butyrate buffer, which was added in order to stabilize the samples. Butyrate appears to act as an inhibitor.

f

The reported g-values are those of a “butyrate-inhibited” form of the enzyme.

g

Sugiura, Y.; Kuwahara, J.; Nagasawa, T.; Yamada, H. J. Am. Chem. Soc. 1987, 109, 5848- 5850.

h

Brennan, B. A.; Cummings, J. G.; Chase, D. B.; Turner, I. M., Jr.; Nelson, M. J. Biochemistry 1996, 35, 10068–10077.