MARV virus detection and measurement. For cynomolgus and rhesus macaques, MARV and RAVV titers were quantitatively measured in blood and tissues by using plaque assays. For African green monkeys, only the viral titers determined by using the TCID50 assay are reported; titers determined in guinea pigs by using the end-point dilution method are not. Viral load data in humans were available only for 4 patients from the 1967 and 1975 outbreaks.91 To confirm active viral infection, human specimens have been tested by using animal inoculation or immunocytochemistry historically or PCR, immunohistochemistry, or ELISA more recently.