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. 2015 Jun 19;21:1774–1780. doi: 10.12659/MSM.893458

Figure 3.

Figure 3

miR-15b regulates IL-15 expression by directly targeting its 3′-UTR. (A) Predicted binding site between miR-15b and 3′-UTR of IL-15. The mutant 3′-UTR of IL-15 without specific binding site is also given. (B, C) (B) HEK293T cells or (C) C2C12 cells transfected with 100nM miR-15b mimics had significantly increased miR-15b. (D, E) (D) HEK293T cells or (E) C2C12 cells were co-transfected with 100 nM of miR-15b mimics or NC oligos and 200 ng plasmids (Luc-IL-15-WT or Luc-IL-15-MUT). miR-15b could significantly abrogate the luciferase activity of Luc-IL-15-WT, but had no inhibitive effect on Luc-IL-15-MUT. Error bars indicate SD. * P<0.05, ** P<0.01, *** P<0.001.