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. 2015 Mar 19;4(6):e1008355. doi: 10.1080/2162402X.2015.1008355

Figure 4.

Figure 4.

Tumor-derived pDCs induce the production of IL-10 by Tr1 cells through ICOS-ligand-ICOS signaling. (A) Blood pDCs isolated from healthy donors after overnight culture in the presence of lysates from TFL (TFLL-pDCs) or tumor tissue (TL-pDCs) were analyzed for the expression of ICOS-L. pDCs exposed to tissue lysates were used to stimulate autologous naive CD4+ T cells. The expression of IL-10 (B) and CD49b and Lag-3 (C) were analyzed on CD4+ T cells after co-culture with pDCs and re-stimulation with PMA/Ionomycin. IL-10 production was analyzed in CD3+CD4+FoxP3−CD49b+LAG-3+ T cells. To evaluate the impact of ICOS-ICOS-L signaling, cells were co-cultured in the presence of 50 ug/mL of control isotype antibody or anti-ICOS-L neutralizing antibody. Values are means ± SEM, *p < 0.05, **p < 0.01. Red dots represent HCC lysates and blue open dots LM-CRC lysates. (D) Pearson correlation analysis of the expression of ICOSL on pDCs cultured with medium, TFLL or TL, and the percentage of IL-10+ Tr1 cells detected after co-culture.