Table 3.
Antibodies used for immunofluorescence staining
| Antigen | Clone | Dilution | Isotype | Label | Source |
|---|---|---|---|---|---|
| slan | DD2 | 1:100 | IgM | unlabeled | Our laboratory |
| CD3 | SK7 | 1:10 | IgG1 | APC-H7 | BD Biosciences |
| CD3 | BW264/56 | 1:50 | IgG2a | PE/FITC | Miltenyi biotec |
| CD4 | M-T466 | 1:100 | IgG1 | FITC | Miltenyi biotec |
| CD4 | RPA-T4 | 1:50 | IgG1 | PE | BD Biosciences |
| CD8 | B9.11 | 1:100 | IgG1 | FITC | Beckman Coulter |
| CD11c | B-ly6 | 1:10 | IgG1 | APC | BD Biosciences |
| CD16 | 3G8 | 1:10 | IgG1 | FITC | BD Biosciences |
| CD40 | 5C3 | 1:10 | IgG1 | PE | BD Biosciences |
| CD45 | 2D1 | 1:10 | IgG1 | PerCP | BD Biosciences |
| CD45RA | HI100 | 1:50 | IgG2b | FITC | BD Biosciences |
| CD56 | NCAM16.2 | 1:50 | IgG | PE | BD Biosciences |
| CD80 | L307.4 | 1:10 | IgG1 | PE | BD Biosciences |
| CD83 | HB15e | 1:10 | IgG1 | FITC | BD Biosciences |
| CD86 | 2331 | 1:10 | IgG1 | FITC | BD Biosciences |
| B7-DC (CD273) | MIH18 | 1:10 | IgG1 | PE | BD Biosciences |
| B7-H1 (CD274) | MIH1 | 1:10 | IgG1 | PE | BD Biosciences |
| B7-H3 (CD276) | FM276 | 1:10 | IgG2b | FITC | Miltenyi biotec |
| B7-H4 | MIH43 | 1:10 | IgG1 | PE | BD Biosciences |
| HLA-DR | G46-6 | 1:50 | IgG2a | APC | BD Biosciences |
| HLA-DR | TU36 | 1:10 | IgG2b | FITC | BD Biosciences |
| CCR7 | 3D12 | 1:10 | IgG2a | PE | BD Biosciences |
| ILT3 | 293623 | 1:10 | IgG2a | FITC | R&D Systems |
| ILT4 | 287219 | 1:10 | IgG2a | FITC | R&D Systems |
| IFNγ | 4S.B3 | 1:40 | IgG1 | FITC | BD Biosciences |
| IL-4 | 8D4-8 | 1:40 | IgG1 | PE | BD Biosciences |
| IL-10 | JES3-9D7 | 1:40 | IgG1 | PE | BD Biosciences |
| IL-12 | C11.5 | 1:40 | IgG1 | PE | BD Biosciences |
| TNF-α | MAb11 | 1:40 | IgG1 | PE | BD Biosciences |
| Other reagents | |||||
| Goat F(ab´)2 anti-mouse IgM | 1:600 | PE/FITC | Beckman Coulter |
Abbreviations: APC: allophycocyanin, CD: cluster of differentiation, FITC: fluorescein isothiocyanate, HLA: human leukocyte antigen, IFN: interferon, IL: interleukin, ILT: immunoglobulin-like transcript, PE: phycoerythrin, PerCP: peridinin chlorophyll protein, slan: 6-sulfo-LacNAc