Table 5.
Antibodies used for immunohistochemistry
| Marker | Source | Type | Manufacturer | Dilution |
|---|---|---|---|---|
| CD3 | Mouse | Monoclonal | Dako (Glostrup, Denmark) | 1:1,600 |
| CD4 | Goat | Polyclonal | R&D Systems (Minneapolis, MN) | 1:100 |
| CD8 | Mouse | Monoclonal | Dako | 1:200 |
| CD20 | Mouse | Monoclonal | Dako | 1:4,000 |
| CD45RO | Mouse | Monoclonal | Dako | 1:4,000 |
| FoxP3 | Mouse | Monoclonal | Abcam (Cambridge, United Kingdom) | 1:2,000 |
| CD68 | Mouse | Monoclonal | Dako | 1:2,000 |
| CD163 | Mouse | Monoclonal | Vector (Burlingame, CA) | 1:100 |
| CCR7 | Rabbit | Monoclonal | Epitomics (Burlingame, CA) | 1:100 |
| CXCL12 | Mouse | Monoclonal | R&D Systems | 1:1,000 |
| CXCR4 | Mouse | Monoclonal | R&D Systems | 1:6,000 |
| IL-7R | Mouse | Polyclonal | Santa Cruz Biotechnology (Santa Cruz, CA) | 1:2,000 |
| IL12R-β2 | Goat | Polyclonal | Santa Cruz Biotechnology | 1:100 |
Abbreviations: FoxP3 = forkhead box P3; IL-7R = interleukin-7 receptor; IL-12Rβ2 = interleukin-12 receptor β2.