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. 2015 Mar 19;4(7):e1014760. doi: 10.1080/2162402X.2015.1014760

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© 2015 The Author(s). Published with license by Taylor & Francis

© Karin Schilbach, Mohammed Alkhaled, Christian Welker, Franziska Eckert, Gregor Blank, Hendrik Ziegler, Marco Sterk, Friederike Müller, Katja Sonntag, Thomas Wieder, Heidi Braumüller, Julia Schmitt5, Matthias Eyrich, Sabine Schleicher, Christian Seitz, Annika Erbacher, Bernd J Pichler, Hartmut Müller, Robert Tighe, Annick Lim, Stephen D Gillies, Wolfgang Strittmatter, Martin Röcken, and Rupert Handgretinger

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 4. — Clonality analysis of αβ T cells treated with FcIL-7, NHS-IL12/FcIL-7, and NHS-IL12/IL-2MAB602. (A) Real-time PCR-based detection of various immune markers in tumor homogenates. Expression of the target gene was normalized to expression of human CD45. (B) Expression of 25 TRBV segments determined by CDR3-size spectratyping. Filled squares indicate expression of up to 12 fragments. Each vertical lane represents 1 mouse; dark squares indicate TRBV segments chosen for CDR3 sequence analysis. (C) CDR3 region protein sequences of selected TRBV segments; bold amino acid codes mark homologous sequences. (D) Expression ratios of T-bet/RORC and Foxp3/CD40L in tumor homogenates (n=4). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.