Figure 1.

Modifications of the T cell infiltrate in the BM of Vk*MYC mice during disease progression. (A and B) The frequency of CD8⁺ and CD4⁺ T cells was assessed in the BM of Vk*MYC mice and age-matched WT littermates by flow cytometry after staining with the indicated mAbs. Each dot represents an individual mouse. (C–E) Cells were also analyzed by intracellular cytokine production assay upon incubation with PMA and Ionomycin for 4 h at 37°C. BFA was added during the last 3 h of stimulation. Dead cells were excluded by live/dead staining. Aggregated data from five independent experiments are reported as mean ± SE. Statistical analyses (Student's t test): *p < 0.05; **p < 0.01; ***p < 0.001.