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. 2015 Feb 3;4(5):e1003011. doi: 10.1080/2162402X.2014.1003011

Figure 3.

Figure 3.

γδ TCR signaling increases membrane IL-18Rβ chain expression. (A) A Vδ1 γδ T polyclonal cell line, Vγ9Vδ1 cell clone, or B-cell lymphoma BEBV cell line were incubated with various concentrations of anti-CD3 for 24 h at 37°C, and immunostained with PE-conjugated anti-IL-18Rβ or IgG-PE control (for the Vδ1 γδ T polyclonal cell line). The expression of membrane IL-18Rβ chain was determined by flow cytometry within the healthy cell population. IL-18Rβ-PE+ cells are shown in the gate with the percentage of positive cells as indicated. (B) Bar graph summarizing IL-18Rβ-PE+ cells (%) from various Vδ2neg γδ T-cell clones (Vγ9Vδ1) and polyclonal cell lines (Vδ1, Vδ1+3, Vδ5) after incubation with various concentrations of anti-CD3. Data are representative of at least 3 independent experiments with similar results (mean of at least 5.103 cells).