Figure 2.

Maternal immunization against an oncoantigen, but not an unrelated antigen, delayed mammary carcinoma onset in neu⁺ offspring. (A) Detection of vaccination-induced anti-Amot antibodies in sera and milk of control (white) and pAmot (black) mothers and in the sera of their 4-week-old offspring. ***, P ≤ 0.0003, Student's t-test. (B) Tumor incidence in control (dotted black line, n = 11) and pAmot (continuous black line, n = 18) neu⁺ offspring. Data are representative of 3 independent experiments. **, p = 0.001, Mantel–Haenszel Log-rank test. (C) Western blotting analysis of β-galactosidase protein. Sera from control and LacZ mothers and their offspring were used as primary antibodies, recombinant β-galactosidase as positive control, and HSP90 protein as loading control. (D) Tumor incidence in control (dotted black line, n = 10) and LacZ (continuous black line, n = 8) neu⁺ offspring.