Table 1.

(B) NY-ESO-1-specific T cells after GMP-grade isolation and expansion

	Start of culture	Post IFN-γ isolation		Post expansion
Donor No.	Cell count	Cell count	Purity CD3+/IFNγ+ [%]	Expansion factor	Absolute CD4+	Absolute CD8+	Specific CD4 [%]	Specific CD8 [%]
1	500 × 106	5.4 × 106	44.63	351	*1600 × 106	*131 × 106	28.04	7.82
7	500 × 106	20.0 × 106	54.31	19	*224 × 106	*118 × 106	14.99	0.98
4	400 × 106	3.3 × 106	59.26	212	31 × 106	41 × 106	37.79	0.02
8	1000 × 106	2.4 × 106	82.53	79	*38 × 106	*29.15 × 106	25.65	0.50

^*Extrapolated cell count, assuming that all cells are used for expansion.

Absolute number and frequencies of T cells after large scale GMP grade enrichment and expansion of PBMCs from 4 donors: Cells were generated according to the protocol and analyzed at different timepoints. Asterics indicate preparations for which not all available cells were expanded after IFNγ-based enrichment. In these cases the cell number was extrapolated calculated by the expansion factor.