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. 2015 Apr 1;4(7):e1016699. doi: 10.1080/2162402X.2015.1016699

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© 2015 The Author(s). Published with license by Taylor & Francis

© Tomomi Toubai, Hou Guoqing, Corrine Rossi, Nathan Mathewson, Katherine Oravecz-Wilson, Emily Cummings, Julia Wu, Yaping Sun, Sung Choi, and Pavan Reddy

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 5. — Ikaros deficiency in both hematopoietic APCs and leukemia cells ameliorates GVL responses. WT B6Ly5.2 animals were lethally irradiated with 11 Gy and infused with 5 × 10⁶ BM cells and 5 × 10⁶ splenocytes from syngeneic Ly5.1 WT B6 or Ik^−/− B6 donors. These animals [B6→B6Ly5.2] or [IkDN^+/−→B6Ly5.2]) were used as recipients 4 months later. Mice were irradiated with 9 Gy and transplanted with 1 × 10⁶ CD90⁺ T cells together with 5 × 10⁶ BM cells from either syngeneic B6 or allogeneic MHC-matched or multiple miHA-mismatched C3H.sw donors concurrently with syngeneic P185 (Ik WT tumor) or Ik6 (Ik DN tumor). (A) Tumor mortality data for P185 200/mouse. (•) B6→[B6→B6Ly5.2] (n = 8), (Ë) C3H.sw→[B6→B6 Ly5.2] (n = 16), (–) C3H.sw→[Ik^−/−→B6Ly5.2] (n = 14). Data are combined from 3 independent experiments. (B) CTL assay. Donor CD8⁺ T cells were isolated from spleen (n = 3–4) at day 14 following allo-HCT and used as effector T cells against P185 tumor for⁵¹Cr-release assay. One representative dataset from 3 independent experiments is shown. Data are given as mean + standard deviation. (C) Tumor mortality data of Ik6 200/mouse. (•) B6→[B6→B6Ly5.2] (n=8), (Ë) C3H.sw→[B6→B6 Ly5.2] (n = 16), (–) C3H.sw→[Ik^−/−→B6Ly5.2] (n = 15). Data are combined from 3 independent experiments. (D) CTL assay. Donor CD8⁺ T cells were isolated from spleen (n = 3–4) at day 14 following allo-HCT and used as effector T cells against Ik6 tumor for⁵¹Cr-release assay. One representative dataset from 3 independent experiments is shown. Data are given as mean + standard deviation.