Fig 2. Transgenes used for generating nervonic acids in camelina seeds.
(A). pBinGlyRed2-LaKCS contains seed-specific cassettes for the expression of LaKCS gene. (B). pBinGlyRed2-LaKCS+AtKCR contains seed-specific cassettes for the expression of LaKCS and AtKCR genes. (C). pBinGlyRed2-LaKCS+AtHCD contains seed-specific cassettes for the expression of LaKCS and AtHCD genes. (D). pBinGlyRed2-LaKCS+AtECR contains seed-specific cassettes for the expression of LaKCS and AtECR genes. (E). pBinGlyRed2-LaKCS+AtKCR+AtHCD contains seed-specific cassettes for the expression of LaKCS, AtKCR and AtHCD genes. Selection was accomplished with a DsRed florescence marker. Constitutive and seed-specific promoters, 3’UTR sequences and arrangements of cassettes are also shown.