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. 2015 Mar 6;2(5):421–431. doi: 10.1016/j.ebiom.2015.03.002

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© 2015 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 2 — Absence of AKT1 in-frame duplications from a series a tumor and cancer cell line genomic DNA samples.

A) Exon-3 amplicons in 10 adult-type GCTs. Control + stands for exon 3 in T14 (carrying the insertion, note the bands at 154 bp and 190 bp), − denotes PCR control (without DNA). Electrophoresis migration was performed in 2% agarose gels of 24 cm to ensure allelic discrimination by size. B) Exon-3 amplicons in colorectal cancer (CRC) samples. C) Exon-3 amplicons in the 60 cell line panel of the National Cancer Institute. Control + stands for the amplicon in T13 (carrying the insertion, note the bands at 144 bp and 192 bp), − denotes PCR control (without DNA). Only one band having the wild-type length was obtained in all the samples tested thus far. Cell lines: 1: K-562; 2: MOLT-4; 3: CCRF-CEM; 4: RPMI-8226; 5: HL-60(TB); 6: SR; 7: SF-268; 8: SF-295; 9: SF-539; 10: SNB-19; 11: SNB-75; 12: U251; 13: BT-549; 14: HS 578T; 15: MCF7; 16: NCI/ADR-RES; 17: MDA-MB-231/ATCC; 18: MDA-MB-435; 19: T-47D; 20: COLO 205; 21: HCC-2998; 22: HCT-116; 23: HCT-15; 24: HT29; 25: KM12; 26: SW-620; 27: A549/ATCC; 28: EKVX; 29: HOP-62; 30: HOP-92; 31: NCI-H322M; 32: NCI-H226; 33: NCI-H23; 34: NCI-H460; 35: NCI-H522 (empty tube); 36: LOX IMVI; 37: M14; 38: MALME-3M; 39: SK-MEL-2; 40: SK-MEL-28; 41: SK-MEL-5; 42: UACC-257; 43: UACC-62; 44: IGR-OV1; 45: OVCAR-3; 46: OVCAR-4; 47: OVCAR-5; 48: OVCAR-8; 49: SK-OV-3; 50: DU-145; 51: PC-3; 52: 786–0; 53: A498; 54: ACHN; 55: CAKI-1; 56: RXF 393; 57: SN12C; 58: TK-10; 59: UO-31; 60: MDA-MB-468.