Table 1. Transgenic mice expressing G127 and V127 human PrP.
| Transgenic mice | PRNP Codon 127-129 genotype | Total HuPrP* expression level (x) | WT† G127 allele expression level (x) | Variant‡ V127 allele expression level (x) | PrP Ratio G127 : V127 |
|---|---|---|---|---|---|
| Parental lines: | |||||
| Tg35c | G127M129/G127M129 | 2 | 2 | - | - |
| Tg152c | G127V129/G127V129 | 6 | 6 | - | - |
| Tg183 | V127M129/V127M129 | 2 | - | 2 | - |
| Tg190 | V127M129/V127M129 | 1 | - | 1 | - |
| F1 crosses: | |||||
| Tg35c × Tg183 | G127M129/V127M129 | 2 | 1 | 1 | 1 : 1 |
| Tg35c × Tg190 | G127M129/V127M129 | 1.5 | 1 | 0.5 | 2 : 1 |
| Tg152c × Tg183 | G127V129/V127M129 | 4 | 3 | 1 | 3 : 1 |
| Tg152c × Tg190 | G127V129/V127M129 | 3.5 | 3 | 0.5 | 6 : 1 |
| Tg152c +/o (Hemi)§ | G127V129 | 3 | 3 | - | - |
WT = wild type
*
PrP expression level is relative to pooled 10% (w/v) normal human brain homogenate
†
Wild type alleles are either G127M129 or G127V129
‡
Variant allele is V127M129
§
Generated by crossing Tg152c with FVB/PrP-null mice