Fig. 8.

Changes in SOAR1 expression alter expression of a subset of genes involved in cold stress response. The mRNA levels in the seedlings of wild-type Col, soar1-2 mutant and the SOAR1-overexpression line OE1 were determined by real time RT-PCR. Seedlings were grown at 20 °C for 2 weeks, and transferred into a chamber at 4 °C for the indicated time. Expression of CBF1/DREB1B, CBF2/DREB1C, CBF3/DREB1A, COR15A, COR15B, COR47, COR414, KIN1, ICE1, SIZ1, MYB15, RD29A, P5SC1, SUS, and FAD2 were analyzed. The gene expression levels were relative units normalized relative to the value from the sample of the wild-type Col plants (as 1). Each value is the mean ± SE of three independent determinations, and different letters indicate significant differences at P < 0.05 (Duncan’s multiple range test) within the same time point