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. 2015 Jun 4;16(1):66. doi: 10.1186/s12931-015-0226-2

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© Tang et al. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Histone modifications of the Notch1 gene promoter in rats at 3 and 9 weeks. a Schematic illustration showing the different enrichment peak (red bar) detected by Medip-chip in the distal promoter of the Notch1 gene and the region analyzed by bisulfite sequencing (BS) (blue bar). ChIP-qPCR analysis of regions proximal to the transcription start site are also indicated (yellow bar). The distribution pattern of four histone modifications at three sites in the proximal promoter (<−1500 bp) of Notch1 at (b) 3 weeks and (c) 9 weeks, analyzed by ChIP/real-time qPCR. Data are presented as mean ± SEM. (PVECs from 9 rats of each group were assessed). *P < 0.05