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. 2015 Jun 29;10(6):e0131961. doi: 10.1371/journal.pone.0131961

Fig 5. CRISPR-induced editing of ARID3B causes loss of ARID3B, and downregulation of ARID3B targets.

Fig 5

(A) Western blot using whole-cell lysate from OVCA429 and OVCA429 ARID3B CRISPR cells, with detection for ARID3B. N = 2. (B) qRT-PCR was performed for EGFR, TRADD, and TNFR2 on total RNA from OVCA429 cells transduced with Cas9 nuclease and a control scrambled CRISPR guide RNA, and OVCA429 cells with ARID3B edited by targeted CRISPR vectors. (C) qRT-PCR was performed for ARID3B on OVCA429-GFP and OVCA429 cells sorted for medium ARID3B-GFP or high ARID3B-GFP expression. (D) qRT-PCR was performed for EGFR, TRADD, TNFR2, TNF, and TRAIL on OVCA429-GFP, OVCA429 medium ARID3B-GFP, and OVCA429 high ARID3B-GFP cells. N = 3. * = p<0.05. ** = p<0.005.