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. 2015 Jun 29;10(6):e0131961. doi: 10.1371/journal.pone.0131961

Fig 7. ARID3B and FZD5 increases cell adhesion to extracellular matrices.

Fig 7

(A) qRT-PCR was used to measure FZD5 expression in OVCA429 and Skov3 cell lines transduced to over-express FZD5, or knock it down with shRNA. (B) (B) Representative images of ECM adhesion assays in OVCA429 and Skov3, 10X original magnification. (C and D) ECM adhesion assays were performed on OVCA429 (C) and Skov3 (D) cells, 6xHis-ARID3B expressing cells (dark gray bars) and FZD5 transduced cells (light grey bars). OVCA429 cells with ARID3B edited by CRISPR (white bars) are also included. Skov3 cells with elevated ARID3B combined with FZD5 shRNA (white bars) are compared to Skov3 6xHis-ARID3B cells. Data was normalized to Skov3-RFP cells. Statistical comparisons for C and D are relative to the RFP control cell line, unless otherwise indicated. N = 3. * = p< 0.05.