Figure 3.

Heterogeneity of ROS in primary CLL. CLL samples were recovered from cryopreservation, rested for 3 h and then stained with CM-H2DCFDA and anti-CD19, anti-CD5 and anti-CXCR4 antibodies prior to flow cytometric analysis. (A) Representative flow analysis showing (i) CM-H2DCFDA fluorescence in CD19⁺CD5⁺ cells and (ii) CXCR4 expression in “ROS^low” and “ROS^high” sub-populations. (B) Quantitative data for CXCR4 expression in “ROS^low” and “ROS^high” sub-populations from four CLL samples analyzed.