TABLE 2. The glucose-6-phosphatase activities at the physiological glucose-6-phosphate level.
The activities of glucose-6-phosphatase were calculated at the glucose 6-phosphate concentration observed in wild-type cells (~114 μm) using the Vmax and Km in crude microsomes, crude cytosol, and cleared lysates, all normalized to the protein concentration in the cleared lysates. For countin− cells, we used Km <0.1 mm (Table I). Values for glucose-6-phosphatase activity in lysates are from Jang and Gomer (18).
| Activity |
|||
|---|---|---|---|
| smlA − | Wild-type | countin − | |
| nmol/mg protein/min | |||
| Crude microsomes | 0.03 | 0.05 | >0.26 |
| Crude cytosol | 0.12 | 0.06 | >0.035 |
| Cleared lysate | 0.10 | 0.08 | >0.23 |