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The activities of rat liver crude microsomes resuspended in PB (control), WT S>10K, or countin− S>10K were measured (Fig. 4). The Vmax and Km values were calculated using nonlinear regressions to fit the activity (as a function of the substrate concentration) to the Michaelis-Menten equation.
| Control | WT S>10K | countin− S>10K | |
|---|---|---|---|
|
Vmax (nmol/mg protein/min) |
21.4 | 4.9 | 3.6 |
| Km (mm) | 1.9 | 1.0 | 0.24 |
