Figure 4. CXCR7^ΔEND/ΔEND mice with orthotopic E0771-FL tumors have greater spontaneous metastases.

A) Representative bioluminescence images of metastatic E0771-FL cells in euthanized CXCR7^ΔEND/ΔEND and control mice. B) Graph of total metastases measured in euthanized mice presented as mean values + SEM (n = 8 per group). C) Anatomic distribution of metastasis quantified by bioluminescence imaging of mice immediately after euthanization. Data are presented as mean values + SEM. D) We quantified disseminated E0771-FL cells in bone marrow by ex vivo bioluminescence imaging. Each point shows the percentage of CXCR7^ΔEND/ΔEND and control mice with detectable breast cancer cells in bone marrow in a given experiment (n = 8–10 mice per group per experiment). Horizontal line shows mean values for percent mice with disseminated tumor cells in four independent experiments. **, p < 0.01.