Skip to main content
. Author manuscript; available in PMC: 2015 Aug 17.
Published in final edited form as: Nat Commun. 2015 Feb 17;6:6252. doi: 10.1038/ncomms7252

Figure 1. Screening animals for ZFN-targeted mutation at the Nr2f2 locus.

Figure 1

(A) Tail DNA samples from pups born post-microinjection of custom ZFNs targeting exon 2 of the Nr2f2 locus were screened by PCR amplification with primers designed to amplify rat genomic fragments encompassing the ZFN-targeted site. Deviations from the expected product size of 360bp were inferred as genomic DNA from rats with mutations at the Nr2f2 locus. (B) Representative sequencing results from the PCR products shown in panel A detected a 15 bp deletion in the mRNA from mutant rats. Also represented are the corresponding translated peptide sequences as a result of the nucleotide variations.