FIG. 1.
Dynamics of infection of donors' blood cells by tick-borne encephalitis virus (TBEV) strains: (A) in blood cells and (B) in plasma. The whole-blood samples were mock-infected and infected with the Dal strain at a multiplicity of infection (MOI) of 0.01 TCID50/cell (gray columns) and with the P-183 strain at a MOI of 0.01 TCID50/cell (columns with hatching). Virus binding to the blood cells was analyzed at 3, 6, and 24 h post-infection (p.i.). (A) The number of blood cells infected with TBEV strains was determined by indirect immunofluorescence and expressed as the percentage of virus-positive cells. (B) The amount of unbound virus in the plasma samples was determined by titration of samples on pig embryo kidney (PK) cells and expressed as log10 TCID50/mL. The results shown were obtained from 10 independent blood samples (each data point represents the mean±SD).