FIG. 5.

CD69, CD25, and CD95 expression on the lymphocyte subpopulations after infection of blood cells by TBEV strains. The whole-blood samples were mock-infected and infected with TBEV strains (P-183 and Dal) at a MOI of 0.01 TCID₅₀/cell and analyzed at 24 h p.i. by flow cytometry for expression of CD69, CD25, and CD95 within lymphocyte subpopulations (NK cells, CD4+, and CD8+ T-lymphocytes). The results obtained from 10 independent blood samples and presented as mean percentage of cells expressing markers (each data point represents the mean±SD of results) are shown. Uncoated (white) columns, % of cells expressing CD69; gray, % of cells expressing CD25; black, % of cells expressing CD95. *Significance of the differences between the parameters of TBEV-infected and mock-infected cells (p≤0.05).