FIG. 1.

Murine cytomegalovirus/cyclophosphamide (MCMV/CY) mice develop significant pneumonitis preceded by a transient increase in viral loads compared to MCMV alone. Wild type recipient mice were infected with intranasal MCMV with or without concomitant CY administration. (A)–(E) Representative day 14 histopathology from MCMV/CY (A: 10×; D: 40×), MCMV alone (B: 10×; b: 40×), and CY-treated mice (C); lung sections were fixed, stained with hematoxylin and eosin as per Materials and Methods. (F) Mean lung histopathology scores with bars representing mean values±standard error of the mean (SEM) for treatment group, with p-values determined by t-test. (G) Viral load counts as quantified by plaque forming units on days 3, 7, 14, 21, and 28. Bars represent mean values±SEM of viral loads for treatment group, with p-values determined by t-test for paired samples and Kruskal–Wallis test for multiple groups. Results represent pathology and counts from individual mice with a minimum of six to eight mice per group. *p<0.05; **p<0.01. Color images available online at www.liebertpub.com/vim