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Unstained brain tissue represents cerebral ischemia. There is no evidence of ischemia in sham animals in the left column (A), small cortical ischemia in temporary MCAO animals in the center column (B), and large MCA territory ischemia in permanent MCAO animals in the right column (C). MCA, middle cerebral artery; MCAO, middle cerebral artery occlusion; TTC, 2,3,5-triphenyltetrazolium chloride, n = 5 shams, n = 11 transient MCAO, n = 8 permanent MCAO.
