Fig. 3.

NBD-phosphocholine accumulation in L. amazonensis clones resistant to miltefosine. Wild-type, MF150.3–1 miltefosine resistant line selected in vitro (Coelho et al., 2014) and miltefosine resistant clones were incubated with the fluorescent analogue NBD-PC for 30 min at 25 °C, washed and evaluated by flow cytometry. Non-labelled and labelled wild-type parasites are shown in light and dark gray respectively. MF150.3–1 trace is shown in red and MNNG-MF resistant clones 2, 3, 5, 6, 8, 10, 11, 12, 13 and 15 appear in different shades of blue and green (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.).