Skip to main content
. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Nat Commun. 2014 Aug 28;5:4202. doi: 10.1038/ncomms5202

Figure 2. Inhibition of IRE1α function by MKC9989 in human RPMI 8226 plasmacytoma cells.

Figure 2

(a) Time course analysis of MKC9989 inhibitory function on XBP1 splicing: RT-PCR PAGE analysis (top) and quantification (middle) of XBP1 spliced products from 3 independent experiments shown as +/− s.e.m. Quantification of CD59 transcript levels from the same RNA samples (bottom). (b) Dose response analysis of MKC9989 treatment on XBP1 splicing in human RPMI 8226 plasmacytoma cells. Shown is a representative profile with EC50 calculated as the average of 2 independent experiments.