Table 2.
Homology of non-human primate nAChR coding sequences with human (hg19).
| Gene ID | Amino Acids |
great apes | old world | new world | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chimp (pan- Tro4) |
Gorilla (gor- Gor3) |
Orangutan (ponAbe2) |
Gibbon (nom- Leu3) |
Cyno (mac- Fas5) |
Rhesus (rhe- Mac3) |
Baboon (pap- Ham1) |
Green monkey (chlSab1) |
Marmoset (calJac3) |
Squirrel monkey (saiBol1) |
Bushbaby (otoGar3) |
Mouse (mm10) |
||
| CHRNA2 | 529 | 98.9 | 98.5 | 97.5 | 97.9 | 97.2 | 96.8 | 97.0 | 97.4 | 95.8 | 95.5 | 86.6 | 83.6 |
| CHRNA3 | 505 | 99.6 | 99.4 | 99.8 | 99.6 | 98.8 | 98.4 | 98.8 | 98.8 | 98.6 | 96.3 | 94.8 | 92.9 |
| CHRNA4 | 627 | 98.3 | 98.7 | 97.7 | 97.0 | 96.5 | 96.2 | 96.6 | 92.7 | 96.7 | 87.3 | 92.6 | 85.3 |
| CHRNA5 | 468 | 99.1 | 99.6 | 98.7 | 98.7 | 98.5 | 98.8 | 99.2 | 97.9 | 96.4 | 95.8 | 94.4 | 89.3 |
| CHRNA6 | 494 | 99.6 | 99.1 | 97.6 | 97.8 | 96.4 | 96.4 | 96.0 | 97.2 | 97.0 | 96.9 | 88.3 | 85.6 |
| CHRNA7 | 502 | 100.0 | 99.8 | 98.8 | 99.2 | 99.0 | 99.2 | 98.6 | 99.0 | 98.4 | 99.0 | 96.8 | 93.6 |
| CHRNA9 | 479 | 99.2 | 98.5 | 99.6 | 98.7 | 98.5 | 98.5 | 98.3 | 98.1 | 98.3 | 97.9 | 93.7 | 91.0 |
| CHRNA10 | 466 | 99.1 | 99.1 | 98.9 | 98.7 | 96.7 | 96.7 | 96.9 | 96.9 | 96.7 | 96.0 | 93.8 | 92.6 |
| CHRNB2 | 502 | 100.0 | 100.0 | 99.0 | 99.4 | 99.2 | 99.0 | 99.4 | 99.6 | 98.2 | 98.2 | 94.0 | 94.4 |
| CHRNB3 | 458 | 99.8 | 99.6 | 98.3 | 99.1 | 98.4 | 99.1 | 98.4 | 97.6 | 98.0 | 98.7 | 92.3 | 87.0 |
| CHRNB4 | 498 | 98.1 | 99.0 | 97.5 | 98.2 | 95.7 | 96.1 | 95.3 | 95.9 | 92.0 | 93.3 | 89.5 | 83.1 |
| Average similarity | 99.2 | 99.2 | 98.5 | 98.6 | 97.7 | 97.7 | 97.7 | 97.4 | 96.9 | 95.9 | 92.4 | 88.9 | |
ClustalW alignment of human and non-human primate nAChR coding sequences demonstrates greater than 95% homology with human for the majority of NHP species assessed, compared to an average of 88.9% homology for human with mouse. CHRNB2 and the ancestral subunit, CHRNA7, displayed the greatest homology to human across NHP families. The cynomolgus and rhesus macaques, commonly used for biomedical research, were 99.6% homologous to each other over all subunits (data not shown) and 97.7% homologous to human across nAChR coding sequences. Genomic assembly used shown in parentheses.