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. 2015 Jun 29;14(7):719–725. doi: 10.1128/EC.00073-15

FIG 2.

FIG 2

Self-association of Rgc1 and Rgc2. (A) Coimmunoprecipitation of Rgc2-His with Rgc2-HA. Rgc2-His was tested for coimmunoprecipitation (IP) with Rgc2-HA from extracts (input) of wild-type cells (DL3187) coexpressing both forms from plasmids p2501 and p3391, respectively. Immunoprecipitates were separated by SDS-PAGE and subjected to immunoblot analysis. Controls were from cells that did not express one of the tagged proteins, or in the absence of the IP antibody (−ab). Molecular mass markers (in kilodaltons) are shown on the right. (B) Rgc2 self-association is not affected by hyperosmotic shock. Sorbitol was added to a final concentration of 1.8 M to cultures of wild-type cells (DL3187) coexpressing Rgc2-HA and Rgc2-His and incubated for the indicated times prior to co-IP analysis. (C) Coimmunoprecipitation of Rgc1-HA with Rgc1-Myc. Rgc1-HA was tested for co-IP with Rgc1-Myc from extracts (input) of wild-type cells (DL3187) coexpressing both forms from plasmids p3395 and p3390, respectively. Controls are as in panel A.