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. 2015 Jun 29;14(7):719–725. doi: 10.1128/EC.00073-15

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FIG 3 — Rgc2 exists as a homodimer in vivo. (A) Rgc2-Myc was immunoprecipitated from an extract of rgc1Δ rgc2Δ cells (DL3207) transformed with YEp181-MET25-RGC2-Myc (p3392), and immunoprecipitates were treated with the indicated concentration of DMS to introduce intersubunit cross-links. Samples were subjected to SDS-PAGE separation and immunoblot analysis. Untreated extract (input) control is in the rightmost lane. (B) Rgc2 associates with Rgc1. Rgc1-Myc was immunoprecipitated from an extract of wild-type cells (DL3187) cotransformed with YEp181-MET25-RGC1-Myc (p3390) and pRS316-MET25-RGC2-HA (p3151) and tested for co-IP with Rgc2-HA. Molecular mass markers (in kilodaltons) are shown on the right. Immunoprecipitates were separated by SDS-PAGE and subjected to immunoblot analysis.