TABLE 1.
Yeast strains
| Strain | Relevant genotype | Source or reference |
|---|---|---|
| DL3187 | MATa S288c (BY4741) his3Δ leu2Δ ura3Δ lys2Δ | Research Genetics |
| DL3207 | MATa S288c rgc1Δ::KanMX rgc2Δ::KanMX | 17 |
| DL4062 | MATα S288c rgc1::KanMX rgc2::RGC2-S344A T808A S948A S75A S827A S1021A S1035A-Flag-HPH (RGC2-7A) | 18 |
| DL4070 | MATα S288c rgc1Δ::KanMX rgc2::RGC2-Flag-HPH | 18 |
| DL4110 | MATa S288c rgc1Δ::KanMX rgc2::RGC2-Flag-HPH | This study |
| DL4111 | MATa S288c rgc1::KanMX rgc2::RGC2-S344A T808A S948A S75A S827A S1021A S1035A-Flag-HPH (RGC2-7A) | This study |
| DL4112 | MATa/α S288c rgc1Δ::KanMX/rgc1Δ::KanMX rgc2::RGC2-Flag-HPH/rgc2::RGC2-Flag-HPH | This study |
| DL4113 | MATa/α S288c rgc1Δ::KanMX/rgc1Δ::KanMX rgc2::RGC2-Flag-HPH/rgc2::RGC2-S344A T808A S948A S75A S827A S1021A S1035A-Flag-HPH (RGC2-7A) | This study |
| DL4114 | MATa/α S288c rgc1Δ::KanMX/rgc1Δ::KanMX rgc2::RGC2-S344A T808A S948A S75A S827A S1021A S1035A-Flag-HPH (RGC2-7A)/rgc2::RGC2-S344A T808A S948A S75A S827A S1021A S1035A-Flag-HPH (RGC2-7A) | This study |