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Effect of estrogen and ERα on SH-SY5Y cells invasion and migration. a-d SH-SY5Y cells were treated with E2 (100nM), or ICI-182780 (300nM). Cells were then measured by trans-well assays. The results were showed in the photographs (a and b) and (c) mean ± SD of triplicate independent experiments and have been repeated 3 times with similar numbers. The effect of Estrogen and ERα on ETS-1 targeted genes MMP1 or MMP9 was detected by WB assay (d). *P < 0.05
