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. 2015 Jun 30;15:63. doi: 10.1186/s12896-015-0156-6

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© Wickramarathna et al. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Engineering S. cerevisiae to produce polyunsaturated fatty acids (FAs). Gas chromatography–mass spectrometry analysis of FA methyl esters derived from the phosphatidylcholine fraction of wild-type yeast cultures transformed with the empty pYESBOP vector (A), pYES + F2F3 (B) and pYES + F2F3 + P1 (C) constructs. pYES + F2F3 + P2 produced similar results to that of pYES + F2F3 + P1.