Table 1.
Fatty acid compositions of lipid classes from transgenic S. cerevisae
| Construct | FA composition of PC (mol %) | ||||||
|---|---|---|---|---|---|---|---|
| C16:0 | C16:1 | C16:2 | C18:0 | C18:1 | C18:2 | C18:3 | |
| F2F3 | 5.65 ± 1.28a | 46.55 ± 0.65 | n.d.b | 1.88 ± 0.53 | 43.74 ± 2.69 | 0.64 ± 0.03 | 0.96 ± 0.15 |
| F2F3 + P1 | 7.98 ± 0.12 | 42.97 ± 0.67 | 4.0 ± 0.31 | 4.26 ± 0.12 | 26.78 ± 0.34 | 7.39 ± 0.59 | 6.62 ± 0.35 |
| F2F3 + P2 | 7.13 ± 0.43 | 45.0 ± 0.36 | 1.52 ± 0.17 | 3.50 ± 0.28 | 34.28 ± 2.05 | 4.28 ± 0.45 | 4.26 ± 0.37 |
| EV | 4.88 ± 0.52 | 41.75 ± 1.51 | n.d. | 2.0 ± 0.26 | 51.35 ± 2.29 | n.d. | n.d. |
| FA composition of DAG (mol %) | |||||||
| F2F3 | 12.17 ± 0.00 | 40.15 ± 0.52 | n.d. | 6.68 ± 0.57 | 40.99 ± 0.04 | <0.01 | <0.01 |
| F2F3 + P1 | 14.54 ± 0.01 | 33.55 ± 1.33 | n.d. | 10.20 ± 0.60 | 28.88 ± 0.32 | 6.75 ± 0.33 | 6.06 ± 0.07 |
| F2F3 + P2 | 13.35 ± 0.01 | 36.66 ± 0.09 | n.d. | 8.67 ± 0.45 | 34.32 ± 0.41 | 3.32 ± 0.12 | 3.68 ± 0.00 |
| EV | 10.19 ± 0.33 | 39.33 ± 0.53 | n.d. | 5.42 ± 0.23 | 45.05 ± 0.04 | n.d. | n.d. |
| FA composition of TAG (mol %) | |||||||
| F2F3 | 11.99 ± 0.06 | 51.58 ± 0.67 | n.d. | 4.04 ± 0.18 | 32.38 ± 0.55 | <0.01 | <0.01 |
| F2F3 + P1 | 13.0 ± 0.12 | 42.29 ± 0.63 | 2.56 ± 0.28 | 6.56 ± 0.11 | 24.09 ± 0.55 | 4.57 ± 0.33 | 6.64 ± 0.35 |
| F2F3 + P2 | 12.56 ± 0.07 | 45.58 ± 0.54 | 1.66 ± 0.04 | 5.66 ± 0.17 | 26.93 ± 0.72 | 2.75 ± 0.08 | 4.85 ± 0.16 |
| EV | 10.17 ± 0.15 | 49.17 ± 0.82 | n.d. | 4.03 ± 0.13 | 36.63 ± 0.53 | n.d. | n.d. |
aData represent results from three independent yeast colonies (means ± SE).
bNot detected.
Fatty acid composition of phosphatidylcholine (PC), diacylglycerol (DAG) and triacylglycerol (TAG) from transgenic yeast expressing LuFAD2 (F2) or LuFAD3b (F3) alone, or along with LuPDCT1 (P1) or LuPDCT2 (P2), as well as wild-type yeast transformed with empty pYESBOP vector (EV).