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. 2015 Jun 25;8:346. doi: 10.1186/s13071-015-0950-y

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© Henriques et al. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Differential expression of TcCAT subfamily members as determined by real-time polymerase chain reaction. Quantification of polysome-associated mRNA expression during metacyclogenesis >relative to that in epimastigotes in samples obtained from metacyclogenesis experiment. Ribosomal L9(gray) and histone H2B(black) were used as controls to normalize the expression of polysomal mRNAs encoding TcCAT. EPI, replicating epimastigotes; STR, nutritional stress; 3H, parasites after differentiation for 3 h; 12H, parasites after differentiation for 12 h; 24H, parasites after differentiation for 24 h; and MET, metacyclics