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. 2015 Jun 29;10(6):e0130605. doi: 10.1371/journal.pone.0130605

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© 2015 Kashiwagi et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — (A) Representative immunoblots of SGLT1 in the total membrane fraction from the murine perfused hearts treated with or without ischemia-reperfusion are shown. Total lysates extracted from the murine small intestine and kidney were immunoblotted as positive controls. (B) Densitometric quantitation normalized to the level of control perfused hearts without ischemia-reperfusion is shown (n = 3 each). (C) Immunoblots of SGLT1 in the plasma membrane fraction from the murine perfused hearts with or without ischemia-reperfusion are shown. (D) Densitometric quantitation normalized to the level of control perfused hearts without ischemia-reperfusion is shown (n = 3 each). In both (A) and (C), immunoblots of Na⁺/K⁺ ATPase from the same membrane are shown as a loading control for the membrane fraction.