Figure 2.

Positive control (a) and isotype control (b). For positive control tissues certainly expressing the questionable antigens were stained. Placental tissue was used for GALNT6 staining, Colon tissue was used for GCNT2 and Uterus was chosen for ST6GALNac1 staining. Furthermore appropriate antibody concentrations were determined in the positive controls. For isotype control same tissues as for positive control are used, but primary antibody is replaced by a control serum, thus excluding unspecific binding signals of the primary antibody.