Figure 1. OPN deficiency reduces BLM-induced pulmonary inflammation and fibrosis. (A) Representative photographs of lungs from WT and OPN^-/- mice 21 days after intratracheal instillation of BLM (1.5 mg/kg). Sections were stained with H&E. (B) OPN expression levels in diverse tissues from WT B6 mice were analyzed using RT-PCR. (C) BM chimeras were prepared by irradiation of WT or OPN^-/- mice, followed by T-cell-depleted BM cell reconstitution (BMWT→^WT, BMWT→ OPN^-/-, BM^OPN-/-→^WT, and BM^OPN-/-→OPN^-/-). Two months after BM cell reconstitution, pulmonary fibrosis was induced with BLM. Representative photographs of lungs 21 days after intratracheal instillation with BLM (1.5 mg/kg).