Figure 3. WT and OPN^-/- mice that received an intratracheal instillation of BLM (1.5 mg/kg) were sacrificed to harvest BALF on 10 days. Cells from BALF were stimulated with PMA, and ionomycin, surface markers and intracellular cytokines were detected using flow cytometric analysis. (A) The percentages of CD4⁺ or γδTCR⁺ cells in BALF were detected using flow cytometric analysis. (B) The percentage of CD4⁺ or γδTCR⁺ cells producing IL-17 or IFN-γ in BALF of BLM-exposed WT and OPN^-/- mice. (C) The number of CD4⁺ T cells in BALF. (D) The number of γδ T cells in BALF.