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. 2015 Jul 1;3:96. doi: 10.3389/fbioe.2015.00096

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Copyright © 2015 Gupta, Mohan, Berkland and Detamore.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Calcium content as measured in the microsphere-based scaffolds over weeks 0, 3, and 6. Calcium content in the acellular constructs (TCP and GRADIENT groups) was subtracted from the calcium content in the corresponding cellular constructs. The calcium contents of the TCP and GRADIENT constructs at week 0 are not reported because of insufficient sample size (n < 3). (A) Total calcium content in micrograms per construct. (B) Normalized calcium content in micrograms per micrograms DNA. All values are expressed as the average ± SD (n = 3–5), p < 0.05. ^@Statistically significant change over week 0 value, ^#statistically significant change over its value at previous time point, *statistically significant change over the PLGA group at same time point, ^$statistically significant change over the TCP and GRADIENT groups at same time point, ^&statistically significant change over the TCP group at same time point, ^{^}statistically significant change over the GRADIENT group at same time point, and ^%statistically significant change over the CS group at same time point.