FIGURE 2.

Temporal trends of mitochondrial mechanisms, oxidant regulation, and calcium regulation in the SOD1 G93A transgenic mouse model. The ratio of SOD1 G93A to wild type (SOD1 G93A/wild type) is plotted over time for each experimental measure. For visualization purposes, in vitro data is plotted at -20 days, embryonic data is plotted at -5 days, and in vivo time points are plotted at their corresponding post-natal day of assessment, 0–150 days. A gray dotted line is provided to show the expected wild type or homeostatic value. Trend lines are generated based on a Gaussian average of the normalized data points. (A) Mitochondrial mechanisms (Complex I, COX, enzyme activity, and cytochrome c). (B) Oxidant markers (8OH2′dG, MDA, H₂O₂) and protective heat shock proteins (HSPs). (C) Calcium regulation (entry, sensitivity, sink, and cytosolic concentration).