Figure 1.
Fluorescence-activated cell sorting (FACS) isolation of mouse bone marrow mesenchymal stem cells (BM MSCs). FACS profile showing the gating strategy for the sorting of BM MSCs (CD45−LIN−CD31−CD44−Sca-1+). The CD31−CD45−LIN− cells were first gated within the live cells (PI−), after which the CD44−Sca-1+ cells were gated. Sca, stem cell antigen; LIN, lineage markers; PI, propidium iodide.